MassGeneral Institute of Neurodegenerative Disease                                Massachusetts General Hospital   Harvard Medical School

Brian Bacskai Lab - Optical Imaging Approaches for Alzheimer's Disease

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Serial in-vivo imaging of ceregral amyloid agiopathy (CAA) in a  Tg2576 mouse starting at 10.3 months.  The images shown are at the 4th, 5th and 6th week of imaging.  CAA deposition proceeds mostly through propagation of existing deposits.  Robbins et al.

Amyloid plaques alter the morphology and trajectory of neurites in-vivo.  GFP filled neurites (in green) curve around the plaque (blue, stained with methoxy-XO4, rather than penetrating the plaque.  The arrow indicates a distrophic neurite.  Blood vessels contain Texas Red Dextran.  Spires et al.

A FLIM pseudocolor representation of a senile plaque labeled with fluorescent 3d6 and 10d5 exhibiting flourescent energy transfer (FRET).  Lifetimes are not uniform throughout the plaque, but rather show a reduction at the periphery (red) as compared to the core (blue/green).  Bacskai et al.

F(ab)₂ fragments of 3d6 are equally effective as full length 3d6 at clearing away diffuse amyloid.(green) after three days in the PDAPP mouse model.  Bacskai et al.